Proteoglycans from Bovine Proximal Humeral Articular Cartilage
نویسندگان
چکیده
منابع مشابه
The heterogeneity of cartilage proteoglycans. Isolation of different types of proteoglycans from bovine articular cartilage.
Proteoglycans, proteins, and glycoproteins were extracted from femoral condyle (FC) and metacarpalphalangeal (MP) articular cartilages and fractionated by sequential density gradient sedimentation under dissociative conditions. The major high density proteoglycan (PG-I) accounted for 74% of the total proteoglycan extracted from the MP cartilage and 79% of that from the femoral condyle. These pr...
متن کاملIsolation and partial characterization of low density proteoglycans from bovine articular cartilage.
Low density proteoglycans (PG-III) were isolated from bovine articular cartilage by extraction with 4 M guanidinium chloride followed by sedimentation in a dissociative CsCl density gradient and fractionation by chromatography on DEAE-cellulose and Sepharose CL-6B columns. The PG fractions obtained were analyzed to determine the amino acid composition, the content of sulfate and carbohydrate co...
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Two species of dermatan sulfate proteoglycans, called DS-PGI and DS-PGII, have been isolated from mature bovine articular cartilages. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis at low ionic strength in 0.01 M phosphate the dermatan sulfate proteoglycans appeared as a single polydisperse species whose molecular weight ranged from 80,000 to 140,000. The dermatan sulfate proteogl...
متن کاملCharacteristics of the nonaggregating proteoglycans isolated from bovine nasal cartilage.
Purified proteoglycans from bovine nasal cartilage (the Al fraction) were separated by sedimentation velocity centrifugation into a fraction enriched in aggregates (Al-Sl) and a fraction of nonaggregated proteoglycans (Al-S2), containing about 85% and 15% of the starting material, respectively. Both fractions were centrifuged in a dissociative CsCl density gradient to remove non-proteoglycan pr...
متن کاملChondrocyte-mediated depletion of articular cartilage proteoglycans in vitro.
The degradation of proteoglycan was examined in cultured slices of pig articular cartilage. Pig leucocyte catabolin (10 ng/ml) was used to stimulate the chondrocytes and induce a 4-fold increase in the rate of proteoglycan loss from the matrix for 4 days. Material in the medium of both control and depleted cultures was mostly a degradation product of the aggregating proteoglycan. It was recover...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43981-1